5ZO2

Crystal structure of mouse nectin-like molecule 4 (mNecl-4) full ectodomain in complex with mouse nectin-like molecule 1 (mNecl-1) Ig1 domain, 3.3A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97776
Spacegroup name	P 3 2 1
Unit cell lengths	207.483, 207.483, 52.937
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.840 - 3.290
R-factor	0.246
Rwork	0.243
R-free	0.30200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1z9m
RMSD bond length	0.011
RMSD bond angle	1.621
Data reduction software	XDS
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.836	3.490
High resolution limit [Å]	3.290	3.290
Rmerge	0.224	1.074
Number of reflections	19407	3096
<I/σ(I)>	6.96	1.64
Completeness [%]	97.2	97.6
Redundancy	5.68	5.74
CC(1/2)	0.990	0.613

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	0.2M calcium acetate, 0.1M HEPES Sodium Salt ,pH7.5, 10% PEG 8000