5ZNR
Crystal structure of PtSHL in complex with an H3K27me3 peptide
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-22 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| 格子定数 [Å] | 43.717, 110.315, 58.643 |
| 格子定数 [度] | 90.00, 96.64, 90.00 |
精密化法
| 残基 | 40.051 - 3.202 |
| R因子 | 0.209 |
| Rwork | 0.206 |
| R-free | 0.23210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5znp |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.010 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.156 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 3.310 |
| 分解能 [Å] (高) | 3.200 | 3.200 |
| Rmerge_l_obs | 0.190 | 0.786 |
| 独立反射数 | 9089 | 904 |
| <I/σ(I)> | 5.4 | 1.2 |
| 完全性 [%] | 99.7 | 99.8 |
| 冗長性 | 3.8 | 3.9 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 10.5 | 277 | 0.2 M lithium sulfate, 2.0 M ammonium sulfate, 0.1 M CAPS, pH 10.5 |
