5ZNM
Colicin D Central Domain and C-terminal tRNase domain
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| 格子定数 [Å] | 116.352, 146.115, 45.763 |
| 格子定数 [度] | 90.00, 102.49, 90.00 |
精密化法
| 残基 | 44.842 - 1.850 |
| R因子 | 0.1737 |
| Rwork | 0.172 |
| R-free | 0.21230 |
| Structure solution method | SAD |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.009 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.973 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 1.880 |
| 分解能 [Å] (高) | 1.850 | 1.850 |
| 独立反射数 | 63995 | |
| <I/σ(I)> | 14.1 | 1.8 |
| 完全性 [%] | 96.4 | 93.9 |
| 冗長性 | 5.1 | 3.8 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 0.1 M HEPES sodium pH 7.5, 2.5% v/v Polyethylene glycol 400, 2.2 M ammonium sulfate |
