5ZHX
Crystal structure of SmgGDS-558 and farnesylated RhoA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-10-10 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.90000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 93.320, 181.770, 205.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 136.100 - 3.500 |
| R-factor | 0.25278 |
| Rwork | 0.250 |
| R-free | 0.30625 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.420 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 136.100 | 3.590 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Number of reflections | 44892 | |
| <I/σ(I)> | 10.4 | |
| Completeness [%] | 99.9 | |
| Redundancy | 43.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 283 | 0.2M Sodium malonate, 20% PEG 3350 |
