5YNB
Crystal structure of MERS-CoV nsp16/nsp10 complex bound to Sinefungin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-03 |
Detector | DECTRIS PILATUS3 R CdTe 300K |
Wavelength(s) | 0.9778 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 66.325, 70.206, 119.198 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.213 - 1.980 |
R-factor | 0.1893 |
Rwork | 0.187 |
R-free | 0.23050 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3r24 |
RMSD bond length | 0.011 |
RMSD bond angle | 0.901 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.230 | |
High resolution limit [Å] | 1.980 | 1.980 |
Rmerge | 0.082 | |
Rmeas | 0.086 | |
Rpim | 0.026 | |
Number of reflections | 79026 | 3837 |
<I/σ(I)> | 19.4 | 3 |
Completeness [%] | 99.8 | 98.8 |
Redundancy | 10.7 | 10.7 |
CC(1/2) | 0.999 | 0.856 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | 10% PEG 5000 ME, 5% Tascimate, pH7.0 |