5YN6
Crystal structure of MERS-CoV nsp10/nsp16 complex bound to SAM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-05-21 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9778 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 66.962, 70.171, 119.804 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.645 - 1.947 |
R-factor | 0.1961 |
Rwork | 0.195 |
R-free | 0.22650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3r24 |
RMSD bond length | 0.018 |
RMSD bond angle | 1.292 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.570 | |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.065 | |
Rmeas | 0.068 | |
Rpim | 0.020 | |
Number of reflections | 83890 | 4132 |
<I/σ(I)> | 20.9 | 2.9 |
Completeness [%] | 99.5 | 99.4 |
Redundancy | 11.9 | 11 |
CC(1/2) | 0.999 | 0.888 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | 10% PEG 5000 ME, 5%Tascimate, pH7.0 |