5YBE
Structure of KANK1/KIF21A complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97538 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.513, 51.958, 136.984 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.395 - 2.111 |
| R-factor | 0.1836 |
| Rwork | 0.180 |
| R-free | 0.24560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hbd |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.215 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX (1.10_2155) |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 5.700 | 2.100 |
| Rmerge | 0.104 | 0.060 | 0.422 |
| Rmeas | 0.118 | 0.068 | 0.517 |
| Rpim | 0.054 | 0.031 | 0.292 |
| Number of reflections | 14123 | 857 | 484 |
| <I/σ(I)> | 5.7 | ||
| Completeness [%] | 92.2 | 97.9 | 65.2 |
| Redundancy | 4.3 | 4.7 | 2.5 |
| CC(1/2) | 0.996 | 0.761 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 0.1M sodium formate pH8.0, 14% PEG3,350 |
