5Y85

Structure-based Insights into Self-Cleavage by a Four-way Junctional Twister-Sister Ribozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-01-01
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.987
Spacegroup name	P 21 21 2
Unit cell lengths	91.182, 109.304, 41.739
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.852 - 2.001
R-factor	0.2189
Rwork	0.218
R-free	0.23740
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	0.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.000
Number of reflections	28779
<I/σ(I)>	16.4
Completeness [%]	99.7
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M NaOAc, pH 5.2, 0.15M CaCl2, 18-22% iso-propanol or MPD