5XZV
Crystal structure of Rad53 1-466 in complex with AMP-PNP
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2016-07-20 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 32 2 1 |
| 格子定数 [Å] | 116.690, 116.690, 140.804 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 28.880 - 3.100 |
| R因子 | 0.273 |
| Rwork | 0.272 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.011 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.668 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.4_1496) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 30.000 | 3.210 |
| 分解能 [Å] (高) | 3.100 | 3.100 |
| Rmerge_l_obs | 0.064 | 0.848 |
| 独立反射数 | 20517 | |
| <I/σ(I)> | 16.3 | |
| 完全性 [%] | 99.6 | 100 |
| 冗長性 | 11 | 11.1 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 100MM CACODYLATE BUFFER, 0.4M NACL, 1.5M (NH4)2SO4 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 100MM CACODYLATE BUFFER, 0.4M NACL, 1.5M (NH4)2SO4 |
