5XGQ
Crystal structure of apo form (free-state) Mycobacterium tuberculosis methionyl-tRNA synthetase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | AREA DETECTOR |
Collection date | 2015-08-16 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.000040 |
Spacegroup name | P 1 |
Unit cell lengths | 49.983, 72.727, 78.376 |
Unit cell angles | 98.90, 90.05, 98.47 |
Refinement procedure
Resolution | 48.686 - 1.899 |
R-factor | 0.2035 |
Rwork | 0.202 |
R-free | 0.23660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2x1l |
RMSD bond length | 0.003 |
RMSD bond angle | 0.589 |
Data reduction software | XDS |
Data scaling software | XDS |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.686 | 2.010 |
High resolution limit [Å] | 1.899 | 1.900 |
Rmerge | 0.094 | 0.723 |
Number of reflections | 158725 | 27608 |
<I/σ(I)> | 6.68 | 0.97 |
Completeness [%] | 93.1 | |
Redundancy | 1.68 | 1.53 |
CC(1/2) | 0.993 | 0.586 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6.1 | 293 | 0.1M calcium acetate, 0.1M sodium cacodylate, 16%PEG8000 |