5X9H
Crystal structure of the Mg2+ channel MgtE in complex with ATP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-10-31 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9791 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 135.330, 85.651, 156.558 |
Unit cell angles | 90.00, 100.02, 90.00 |
Refinement procedure
Resolution | 50.329 - 3.598 |
R-factor | 0.2533 |
Rwork | 0.250 |
R-free | 0.28320 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.519 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.10_2155)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.329 |
High resolution limit [Å] | 3.598 |
Number of reflections | 20361 |
<I/σ(I)> | 15.09 |
Completeness [%] | 98.5 |
Redundancy | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 293 | 9% PEG 4000, 0.2 M MgCl2, and 0.05 M MES pH 6.5, 10 mM ATP |