5X8Y
A Mutation identified in Neonatal Microcephaly Destabilizes Zika Virus NS1 Assembly in vitro
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-04-10 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97853 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 63.934, 100.808, 110.720 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.652 - 2.817 |
R-factor | 0.2148 |
Rwork | 0.210 |
R-free | 0.25960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4o6c |
RMSD bond length | 0.004 |
RMSD bond angle | 0.691 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.880 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.134 | 0.653 |
Rpim | 0.043 | |
Number of reflections | 8937 | |
<I/σ(I)> | 15.9 | 2.8 |
Completeness [%] | 96.4 | 96.4 |
Redundancy | 9.6 | 9.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M MES monohydrate pH 6.0, 14% (w/v) Polyethylene glycol monomethyl ether 2,000, 18% (v/v) 2-Propanol |