5WWX
Crystal structure of the KH2 domain of human RNA-binding E3 ubiquitin-protein ligase MEX-3C complex with RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-12-12 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 74.611, 74.611, 32.535 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.367 - 2.000 |
R-factor | 0.1724 |
Rwork | 0.170 |
R-free | 0.21800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5www |
RMSD bond length | 0.008 |
RMSD bond angle | 0.923 |
Data reduction software | MOSFLM |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 2.070 |
High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
Rmerge | 0.108 | 0.048 | 0.534 |
Number of reflections | 6609 | ||
<I/σ(I)> | 3.6 | ||
Completeness [%] | 99.6 | 98 | 99.8 |
Redundancy | 7.6 | 7 | 7.9 |
CC(1/2) | 0.998 | 0.904 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.04M Magnesium acetate tetrahydrate, 0.05M Sodium cacodylate trihydrate pH 6.0, 30% v/v (+/-)-2-Methyl-2,4-pentanediol |