5WWU

Crystal Structure of HLA-A*2402 in complex with 2009 pandemic influenza A(H1N1) virus and avian influenza A(H5N1) virus-derived peptide H1-25

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-03-10
Detector	SDMS
Wavelength(s)	0.97774
Spacegroup name	P 21 21 21
格子定数 [Å]	71.752, 79.099, 88.100
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	45.506 - 2.794
R因子	0.2275
Rwork	0.225
R-free	0.28420
結合長の平均二乗偏差(RMSD) [Å]	0.003
結合角の平均二乗偏差(RMSD) [度]	0.703
Data reduction software	CNS
Data scaling software	CNS
Phasing software	CNS
Refinement software	PHENIX ((1.10.1_2155))

Quality characteristics

	Overall
分解能 [Å] (低)	50.000
分解能 [Å] (高)	2.794
独立反射数	138842
<I/σ(I)>	35.2
完全性 [%]	94.5
冗長性	10.6

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	6	291	0.1 M MES monohydrate pH 6.0, 14% w/v Polyethylene glycol 4000