5W0P
Crystal structure of rhodopsin bound to visual arrestin determined by X-ray free electron laser
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | FREE ELECTRON LASER |
Source details | SLAC LCLS BEAMLINE CXI |
Synchrotron site | SLAC LCLS |
Beamline | CXI |
Temperature [K] | 273 |
Detector technology | PIXEL |
Collection date | 2014-11-06 |
Detector | CS-PAD CXI-1 |
Wavelength(s) | 1.33 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 109.240, 109.240, 452.640 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.030 - 3.013 |
R-factor | 0.2369 |
Rwork | 0.234 |
R-free | 0.27220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zwj |
RMSD bond length | 0.005 |
RMSD bond angle | 0.845 |
Data reduction software | CrystFEL |
Data scaling software | CrystFEL |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.100 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 76360 | |
<I/σ(I)> | 4.5 | |
Completeness [%] | 72.4 | |
Redundancy | 287 | |
CC(1/2) | 0.988 | 0.233 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 5 | 293 | PEG 400, magnesium acetate, sodium acetate. |