5VSL

Crystal structure of viperin with bound [4Fe-4S] cluster and S-adenosylhomocysteine (SAH)

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-18
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
格子定数 [Å]	58.934, 73.854, 141.872
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	46.065 - 1.972
R因子	0.1571
Rwork	0.155
R-free	0.19970
Structure solution method	SAD
結合長の平均二乗偏差(RMSD) [Å]	0.015
結合角の平均二乗偏差(RMSD) [度]	2.008
Data scaling software	HKL-2000
Phasing software	SHELXD
Refinement software	PHENIX ((1.11.1_2575: 000))

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	50.000	2.040
分解能 [Å] (高)	1.970	1.970
Rmerge_l_obs	0.079	0.578
独立反射数	42331	4223
<I/σ(I)>	12.6	2.4
完全性 [%]	95.0	96.7
冗長性	5.2	4.9

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP		295	100 mM HEPES, pH 6.8 - 7.6, 10 - 30 % (w/v) PEG MME 2000, and 5 mM SAH