5V7S
Crystal structure of Influenza A virus matrix protein M1 (NLS-88E, pH 6.2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-08-20 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 85.612, 133.260, 39.308 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.230 - 2.500 |
| R-factor | 0.2251 |
| Rwork | 0.220 |
| R-free | 0.31150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ea3 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.165 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.230 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.077 | 0.449 |
| Number of reflections | 14742 | 1473 |
| <I/σ(I)> | 12.1 | 3.6 |
| Completeness [%] | 90.7 | 91.9 |
| Redundancy | 5.37 | 5.51 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 293 | Protein solution: 8 mg/ml in 50mM K2HPO4/KH2PO4/H3PO4, 0.2M NaCl, 10mM bME, pH 3.2 Reservoir solution: 0.1M Tris-HCl, pH 8.2, 8% PEG-8K |
