5UI5
Crystal structure of Aquifex aeolicus sigmaN bound to promoter DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-08-15 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.987 |
Spacegroup name | P 32 |
Unit cell lengths | 99.921, 99.921, 123.300 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 14.990 - 3.400 |
R-factor | 0.291 |
Rwork | 0.289 |
R-free | 0.33600 |
Structure solution method | MIR |
RMSD bond length | 0.004 |
RMSD bond angle | 0.907 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | autoSHARP |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 3.520 |
High resolution limit [Å] | 3.400 | 3.400 |
Rmerge | 0.140 | 0.903 |
Number of reflections | 18717 | |
<I/σ(I)> | 20.42 | |
Completeness [%] | 99.0 | 100 |
Redundancy | 13.1 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 50 mM sodium cacodylate, pH 6.5, 0.2 M KCl, 0.1 M Mg-acetate, 14%-18% Polyethylene glycol 8000 |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 50 mM sodium cacodylate, pH 6.5, 0.2 M KCl, 0.1 M Mg-acetate, 14%-18% Polyethylene glycol 8000 |