5TW1
Crystal structure of a Mycobacterium smegmatis transcription initiation complex with RbpA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-03-13 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 133.012, 161.633, 139.211 |
Unit cell angles | 90.00, 107.72, 90.00 |
Refinement procedure
Resolution | 54.905 - 2.760 |
R-factor | 0.2394 |
Rwork | 0.239 |
R-free | 0.27950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xlp |
RMSD bond length | 0.004 |
RMSD bond angle | 0.731 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.000 | 2.859 |
High resolution limit [Å] | 2.760 | 2.760 |
Rmerge | 0.234 | 4.816 |
Number of reflections | 143776 | |
<I/σ(I)> | 22.39 | 0.77 |
Completeness [%] | 99.0 | 100 |
Redundancy | 16.2 | 12.3 |
CC(1/2) | 0.998 | 0.214 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 0.1 M Bis-Tris, pH 6.0, 0.2 M LiSO4, 16% (w/v) polyetheylene glycol 3350, 2.5% (v/v) ethylene glycol |