5TVB
Structure of the TPR oligomerization domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-14 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.97622 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 74.355, 50.669, 94.656 |
Unit cell angles | 90.00, 108.01, 90.00 |
Refinement procedure
Resolution | 41.187 - 2.750 |
R-factor | 0.2474 |
Rwork | 0.241 |
R-free | 0.30270 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.572 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.187 | 2.750 |
High resolution limit [Å] | 2.750 | 2.700 |
Rmerge | 0.256 | |
Number of reflections | 9404 | |
<I/σ(I)> | 39.36 | |
Completeness [%] | 99.2 | 98.9 |
Redundancy | 15.1 | 15 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 7.5 | 299 | 0.1M HEPES 7.5, 30% PEG1000 |