5TGM
Crystal structure of the S.cerevisiae 80S ribosome in complex with the A-site bound aminoacyl-tRNA analog ACCA-Pro
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-10-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 434.730, 286.770, 303.150 |
| Unit cell angles | 90.00, 98.87, 90.00 |
Refinement procedure
| Resolution | 148.723 - 3.500 |
| R-factor | 0.2619 |
| Rwork | 0.261 |
| R-free | 0.31190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.743 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX (1.10.1_2155) |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 200.000 | 3.600 | |
| High resolution limit [Å] | 3.500 | 150.000 | 3.500 |
| Rmerge | 0.614 | 1.443 | |
| Number of reflections | 918642 | ||
| <I/σ(I)> | 4.94 | 1 | |
| Completeness [%] | 99.8 | 99.6 | |
| Redundancy | 5 | ||
| CC(1/2) | 0.988 | 0.363 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | PEG20000 |
