5T5T
AMPK bound to allosteric activator
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-30 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 125.040, 125.040, 403.360 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.930 - 3.460 |
R-factor | 0.212 |
Rwork | 0.211 |
R-free | 0.23100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4qfg |
RMSD bond length | 0.008 |
RMSD bond angle | 0.960 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | BUSTER-TNT (2.11.7) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.570 |
High resolution limit [Å] | 3.450 | 3.450 |
Number of reflections | 25312 | |
<I/σ(I)> | 19.5 | 3.2 |
Completeness [%] | 99.8 | 100 |
Redundancy | 14.8 | 15 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 100 mM trisodium citrate, 500 mM ammonium sulfate, 900 mM lithium sulfate, and 4% glycerol |