5T0F

Crystal structure of the Myc3 N-terminal domain [44-242] in complex with JAZ10 CMID domain [16-58] from arabidopsis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2015-08-14
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9786
Spacegroup name	C 2 2 21
Unit cell lengths	57.321, 65.370, 125.654
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.632 - 2.400
R-factor	0.228
Rwork	0.226
R-free	0.26960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rqw
RMSD bond length	0.007
RMSD bond angle	1.143
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.067	1.000
Number of reflections	9574
<I/σ(I)>	26.1	3.5
Completeness [%]	100.0	99.9
Redundancy	14.3	14.9
CC(1/2)	1.000	0.935

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	0.1 M Tris, pH 6.0, 20% (w/v) polyethylene glycol monomethyl ether 2,000