5S4Q

Tubulin-Z422344882-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-13
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	104.743, 158.302, 179.614
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	118.760 - 2.590
R-factor	0.2272
Rwork	0.226
R-free	0.28150
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	118.760	118.760	2.660
High resolution limit [Å]	2.590	11.580	2.590
Rmerge	0.167	0.072	2.365
Rmeas	0.182	0.079	2.557
Total number of observations	643443
Number of reflections	94960	1182	6952
<I/σ(I)>	6.68	22.69	0.7
Completeness [%]	99.9	98.8	100
Redundancy	6.776	6.355	6.879
CC(1/2)	0.994	0.993	0.413

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine