5Q0C

Human liver fructose-1,6-bisphosphatase 1 (fructose 1,6-bisphosphate 1-phosphatase, E.C.3.1.3.11) complexed with the allosteric inhibitor 1-(5-bromo-1,3-thiazol-2-yl)-3-[5-(4-methoxyphenyl)thiophen-2-yl]sulfonylurea and with fructose-2,6-diphosphate

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2006-06-12
Detector	MAR CCD 165 mm
Wavelength(s)	0.987
Spacegroup name	C 2 2 21
格子定数 [Å]	222.475, 240.518, 138.979
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	29.793 - 2.400
R因子	0.1703
Rwork	0.168
R-free	0.21030
Structure solution method	MOLECULAR REPLACEMENT
結合長の平均二乗偏差(RMSD) [Å]	0.008
結合角の平均二乗偏差(RMSD) [度]	0.960
Refinement software	PHENIX (1.11.1_2575)

Quality characteristics

	Overall
分解能 [Å] (低)	29.793
分解能 [Å] (高)	2.400
独立反射数	141184
完全性 [%]	97.5

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, SITTING DROP			entry deposited much later from experimental date, thus exact crystallization details not known