5Q0A

Human liver fructose-1,6-bisphosphatase 1 (fructose 1,6-bisphosphate 1-phosphatase, E.C.3.1.3.11) complexed with the allosteric inhibitor 1-(5-cyanopyrazin-2-yl)-3-[3-(difluoromethoxy)phenyl]sulfonylurea

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2007-12-21
Detector	MAR CCD 165 mm
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
格子定数 [Å]	68.611, 84.335, 279.525
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	29.145 - 2.300
R因子	0.1884
Rwork	0.186
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
結合長の平均二乗偏差(RMSD) [Å]	0.007
結合角の平均二乗偏差(RMSD) [度]	1.006
Refinement software	PHENIX (1.11.1_2575)

Quality characteristics

	Overall
分解能 [Å] (低)	29.145
分解能 [Å] (高)	2.300
独立反射数	73055
完全性 [%]	99.9

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, SITTING DROP	7	300	0.1M HEPES pH 7.0, 0.1M AMMONIUM ACETATE, 12% PEG 3350