5PZR

Human liver fructose-1,6-bisphosphatase 1 (fructose 1,6-bisphosphate 1-phosphatase, E.C.3.1.3.11) complexed with the allosteric inhibitor 1-(3-chlorophenyl)sulfonyl-3-[3-[3-[(3-chlorophenyl)sulfonylcarbamoylamino]propoxy]propyl]urea

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2006-03-17
Detector	MAR CCD 165 mm
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
格子定数 [Å]	67.306, 83.148, 277.665
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	29.833 - 1.900
R因子	0.1801
Rwork	0.178
R-free	0.21760
Structure solution method	MOLECULAR REPLACEMENT
結合長の平均二乗偏差(RMSD) [Å]	0.007
結合角の平均二乗偏差(RMSD) [度]	0.871
Refinement software	PHENIX (1.11.1_2575)

Quality characteristics

	Overall
分解能 [Å] (低)	29.833
分解能 [Å] (高)	1.900
独立反射数	115654
完全性 [%]	93.5

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, SITTING DROP	7	300	0.1M HEPES pH 7.0, 0.1M AMMONIUM ACETATE, 12% PEG 3350