5O89

Crystal Structure of rsEGFP2 in the fluorescent on-state determined by SFX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SLAC LCLS BEAMLINE CXI
Synchrotron site	SLAC LCLS
Beamline	CXI
Temperature [K]	293
Detector technology	PIXEL
Collection date	2015-05-08
Detector	CS-PAD CXI-1
Wavelength(s)	1.3
Spacegroup name	P 21 21 21
Unit cell lengths	51.670, 62.260, 71.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.737 - 1.700
R-factor	0.1689
Rwork	0.168
R-free	0.19480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dtx
RMSD bond length	0.006
RMSD bond angle	0.973
Data reduction software	CrystFEL (0.6.1)
Data scaling software	CrystFEL (0.6.1)
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2152: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.760
High resolution limit [Å]	1.700	1.700
Number of reflections	26122
<I/σ(I)>	9.2
Completeness [%]	99.8
Redundancy	254

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	8	293	2 M ammonium sulphate, 20 mM NaCl, 120 mM HEPES