5O76
Structure of phosphoY371 c-CBL in complex with ZAP70-peptide and UbV.pCBL ubiquitin variant
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2016-10-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97632 |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 94.790, 101.281, 117.339 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 35.340 - 2.473 |
| R因子 | 0.22 |
| Rwork | 0.218 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4a4b |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.008 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.950 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 101.281 | 2.515 |
| 分解能 [Å] (高) | 2.473 | 2.473 |
| Rmerge_l_obs | 0.112 | 1.155 |
| Rpim | 0.066 | 0.667 |
| 独立反射数 | 38315 | 2007 |
| <I/σ(I)> | 13.9 | 2.1 |
| 完全性 [%] | 93.3 | 99 |
| 冗長性 | 6.5 | 6.9 |
| CC(1/2) | 0.998 | 0.796 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M monosaccharides, 0.1 M Buffer 1 pH 6.5, 50% Precipitant 2, Morpheus screen |
