5O6T

BIRC4 RING in complex with dimeric ubiquitin variant

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	110
Detector technology	PIXEL
Collection date	2016-04-28
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
格子定数 [Å]	35.715, 69.972, 109.747
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	43.180 - 1.570
R因子	0.158
Rwork	0.156
R-free	0.18300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3eb5 1ubq
結合長の平均二乗偏差(RMSD) [Å]	0.010
結合角の平均二乗偏差(RMSD) [度]	1.150
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	69.970	1.577
分解能 [Å] (高)	1.570	1.570
Rmerge_l_obs	0.074	0.890
Rpim	0.042	0.513
独立反射数	39227	2560
<I/σ(I)>	14.9	2
完全性 [%]	100.0	96.3
冗長性	6.4	6.5
CC(1/2)		0.663

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, SITTING DROP	7.6	291	0.1 M NaHEPES pH 7.5, 1.4 M tri-Na citrate