5O5O
X-ray crystal structure of RapZ from Escherichia coli (P32 space group)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.87260 |
| Spacegroup name | P 32 |
| Unit cell lengths | 92.750, 92.750, 156.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.330 - 3.404 |
| R-factor | 0.2041 |
| Rwork | 0.197 |
| R-free | 0.26630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.323 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2747: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.330 | 3.480 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.123 | 0.638 |
| Number of reflections | 20540 | |
| <I/σ(I)> | 11.2 | 1.8 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 3.5 | 3.58 |
| CC(1/2) | 0.995 | 0.695 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.1 M MES, 1.1 M ammonium sulfate, 0.01 M beta-mercaptoethanol, 1 percent glycerol, pH 7.0 |
