5NKK

SMG8-SMG9 complex GDP bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-27
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9785
Spacegroup name	P 32 2 1
Unit cell lengths	110.605, 110.605, 360.266
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.893 - 2.640
R-factor	0.2341
Rwork	0.232
R-free	0.27410
RMSD bond length	0.003
RMSD bond angle	0.707
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHELXD
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall
Low resolution limit [Å]	47.893
High resolution limit [Å]	2.600
Number of reflections	152344
<I/σ(I)>	12.39
Completeness [%]	99.0
Redundancy	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		285	10% PEG3350, 0.1M Tris pH 8.5