5NJ5
E. coli Microcin-processing metalloprotease TldD/E with phosphate bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-01 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9200 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.770, 173.990, 83.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 75.290 - 1.900 |
| R-factor | 0.1406 |
| Rwork | 0.139 |
| R-free | 0.17810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tv9 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.376 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.12) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 75.290 | 75.290 | 1.950 |
| High resolution limit [Å] | 1.900 | 8.500 | 1.900 |
| Rmerge | 0.087 | 0.027 | 0.551 |
| Rmeas | 0.100 | 0.031 | 0.683 |
| Rpim | 0.047 | 0.014 | 0.392 |
| Number of reflections | 136171 | ||
| <I/σ(I)> | 12.3 | ||
| Completeness [%] | 94.1 | 99.1 | 69.1 |
| Redundancy | 4.2 | 4.6 | 3 |
| CC(1/2) | 0.997 | 0.999 | 0.565 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | NULL |
