5N6H
Structure of the membrane integral lipoprotein N-acyltransferase Lnt from E. coli
Experimental procedure
実験手法 | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-09-07 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.99997 |
Spacegroup name | P 21 21 21 |
格子定数 [Å] | 54.200, 142.300, 199.920 |
格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
残基 | 49.099 - 2.900 |
R因子 | 0.2204 |
Rwork | 0.219 |
R-free | 0.24940 |
Structure solution method | SAD |
結合長の平均二乗偏差(RMSD) [Å] | 0.003 |
結合角の平均二乗偏差(RMSD) [度] | 0.728 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | CRANK2 |
Refinement software | BUSTER |
Quality characteristics
Overall | |
分解能 [Å] (低) | 49.100 |
分解能 [Å] (高) | 2.900 |
独立反射数 | 693957 |
<I/σ(I)> | 9.5 |
完全性 [%] | 100.0 |
冗長性 | 19.6 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | LIPIDIC CUBIC PHASE | 293 | 8 % (v/v) MPD 0.1 MES pH 6.0 0.4 M ammonium citrate |