5MV1
Crystal structure of the E protein of the Japanese encephalitis virulent virus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-10 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97776 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 67.090, 33.190, 98.510 |
Unit cell angles | 90.00, 107.46, 90.00 |
Refinement procedure
Resolution | 19.746 - 2.250 |
R-factor | 0.2137 |
Rwork | 0.211 |
R-free | 0.27240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3p54 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.777 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.300 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 21345 | |
<I/σ(I)> | 10.2 | |
Completeness [%] | 98.9 | 98.9 |
Redundancy | 4.4 | 4.6 |
CC(1/2) | 0.990 | 0.380 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 0.1M Tris-HCI, pH 8.0, 16% PEG 1000, 0.3M ammonium acetate |