5L3C
Human LSD1/CoREST: LSD1 E379K mutation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-06 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 0.976256 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 120.368, 180.389, 235.422 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.310 |
| R-factor | 0.19906 |
| Rwork | 0.199 |
| R-free | 0.22324 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2v1d |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.398 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.450 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.130 | 0.800 |
| Number of reflections | 38276 | |
| <I/σ(I)> | 8.5 | 1.2 |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 3.8 | 3.9 |
| CC(1/2) | 0.990 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | Tartrate 1-1.4 M, ADA 100 mM pH 6.5 |
