5L2R
Crystal structure of fumarate hydratase from Leishmania major
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-13 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 65.675, 85.128, 241.476 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 120.738 - 2.054 |
| R因子 | 0.1451 |
| Rwork | 0.144 |
| R-free | 0.17730 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.010 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.066 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.2_1309) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 200.000 | 2.090 |
| 分解能 [Å] (高) | 2.050 | 2.050 |
| Rmerge_l_obs | 0.114 | 0.410 |
| 独立反射数 | 83691 | |
| <I/σ(I)> | 16.5 | 2.8 |
| 完全性 [%] | 98.1 | 97.9 |
| 冗長性 | 5.5 | 5.4 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG 3350 and tacsimate pH5 |
