5L0C
Human metavinculin (residues 959-1134) in complex with PIP2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-28 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 114.520, 100.676, 105.615 |
| Unit cell angles | 90.00, 122.49, 90.00 |
Refinement procedure
| Resolution | 30.450 - 3.100 |
| R-factor | 0.2368 |
| Rwork | 0.235 |
| R-free | 0.26060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3myi |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.130 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 89.080 | 3.270 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rpim | 0.034 | |
| Number of reflections | 18132 | |
| <I/σ(I)> | 15.2 | 2.1 |
| Completeness [%] | 98.2 | 100 |
| Redundancy | 3.7 | 3.8 |
| CC(1/2) | 0.999 | 0.742 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 20 mM citric acid, 80 mM Bis-Tris propane (pH 3.4), and 16% (w/v) PEG 3350 |
