5KDN

ZmpB metallopeptidase from Clostridium perfringens

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-05-28
Detector	RAYONIX MX300HE
Wavelength(s)	0.97950
Spacegroup name	P 21 21 21
格子定数 [Å]	65.890, 68.020, 170.860
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	43.090 - 1.660
R因子	0.15508
Rwork	0.154
R-free	0.18046
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5kdj
結合長の平均二乗偏差(RMSD) [Å]	0.018
結合角の平均二乗偏差(RMSD) [度]	1.863
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0073)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	43.090	1.680
分解能 [Å] (高)	1.660	1.660
Rmerge_l_obs	0.054	0.249
独立反射数	92133
<I/σ(I)>	18.5	5.3
完全性 [%]	99.9	100
冗長性	7.2	6.6

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	PEG 3350, Na/K tartrate, HEPES pH 7.5