5K9M
Crystal Structure of PriB Binary Complex with Product Diphosphate
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-09 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 2 21 21 |
| 格子定数 [Å] | 41.727, 83.020, 95.304 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 38.224 - 1.500 |
| R因子 | 0.1945 |
| Rwork | 0.194 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5inj |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10_2155) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 38.224 | 1.554 |
| 分解能 [Å] (高) | 1.500 | 1.500 |
| Rmerge_l_obs | 0.140 | 1.900 |
| 独立反射数 | 101546 | |
| <I/σ(I)> | 8.59 | 1.7 |
| 完全性 [%] | 99.7 | 99.79 |
| 冗長性 | 1.9 | 1.7 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293.15 | PriB was crystallized by sitting drop vapor diffusion method by 1:1 v/v mixing of 10 mg/mL PriB solution in the presence of 1 mM daptomycin and 1 mM Dimethylallyl diphosphate (DMAPP) with reservoir solution containing 0.1 M Tris pH 8, 28% w/v PEG 4000. The mixed drops of 0.4 uL were equilibrated over a reservoir solution of 50 uL and incubated at 20oC in the dark. |
