5K08
RecA mini intein-Zeise's salt complex
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-26 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.07136 |
| Spacegroup name | P 21 21 2 |
| 格子定数 [Å] | 57.687, 63.930, 37.307 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 32.222 - 1.401 |
| R因子 | 0.1822 |
| Rwork | 0.180 |
| R-free | 0.20990 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 5i0a |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | REFMAC |
Quality characteristics
| Overall | Inner shell | Outer shell | |
| 分解能 [Å] (低) | 100.000 | 100.000 | 1.420 |
| 分解能 [Å] (高) | 1.400 | 3.800 | 1.400 |
| Rmerge_l_obs | 0.088 | 0.071 | 0.913 |
| Rmeas | 0.091 | ||
| Rpim | 0.025 | ||
| Total number of observations | 373588 | ||
| 独立反射数 | 27687 | ||
| <I/σ(I)> | 10.8 | ||
| 完全性 [%] | 99.4 | 98.4 | 96 |
| 冗長性 | 13.5 | 12.3 | 10.4 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION | 8.5 | 293 | 54%-59% (w/v) ammonium sulfate, 0.1 M Tris, pH 8.5, 2 mM Zeise's salt, 2 mM TCEP |
