5JJT

Crystal structure of a type 5 serine/threonine protein phosphatase from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	51.408, 102.705, 95.290
Unit cell angles	90.00, 95.57, 90.00

Refinement procedure

Resolution	45.797 - 2.103
R-factor	0.1886
Rwork	0.186
R-free	0.23090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wao
RMSD bond length	0.003
RMSD bond angle	0.705
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.091
Number of reflections	55086
<I/σ(I)>	12.97
Completeness [%]	96.6
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2	289	0.1M HEPES pH7.2, 22.5% PEG 3350, 20% glycol