5J9Q
Crystal structure of the NuA4 core complex
Experimental procedure
| 実験手法 | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2014-01-10 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 1 2 1 |
| 格子定数 [Å] | 217.857, 190.990, 172.346 |
| 格子定数 [度] | 90.00, 113.56, 90.00 |
精密化法
| 残基 | 29.996 - 3.250 |
| R因子 | 0.2508 |
| Rwork | 0.250 |
| R-free | 0.27310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.004 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.621 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | autoSHARP |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Quality characteristics
| Overall | |
| 分解能 [Å] (低) | 30.000 |
| 分解能 [Å] (高) | 3.250 |
| 独立反射数 | 79960 |
| <I/σ(I)> | 6.9 |
| 完全性 [%] | 79.6 |
| 冗長性 | 3.4 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | EVAPORATION | 289 | 100mM NaCitrate (pH 6.5), 1.79M ammonium sulfate, 5%(w/v) glycerol |
