5J4C
Crystal structure of the Thermus thermophilus 70S ribosome in complex with cisplatin (soaked) and bound to mRNA and A-, P- and E-site tRNAs at 2.8A resolution
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-13 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97900 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 208.980, 446.990, 621.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 152.645 - 2.800 |
| R-factor | 0.2216 |
| Rwork | 0.219 |
| R-free | 0.26810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4y4p |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.889 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 152.645 | 161.643 | 2.870 |
| High resolution limit [Å] | 2.800 | 12.520 | 2.800 |
| Rmerge | 0.175 | 0.025 | 1.465 |
| Rmeas | 0.207 | 0.030 | 1.724 |
| Total number of observations | 4796689 | ||
| Number of reflections | 1384376 | 15471 | 103274 |
| <I/σ(I)> | 6.43 | 24.65 | 0.91 |
| Completeness [%] | 98.3 | 92.6 | 99.5 |
| Redundancy | 3.465 | ||
| CC(1/2) | 0.990 | 0.999 | 0.218 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.6 | 292 | 0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME |
