5I6C
The structure of the eukaryotic purine/H+ symporter, UapA, in complex with Xanthine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-12 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.992 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.090, 173.830, 82.390 |
| Unit cell angles | 90.00, 111.11, 90.00 |
Refinement procedure
| Resolution | 76.861 - 3.700 |
| R-factor | 0.2978 |
| Rwork | 0.296 |
| R-free | 0.32670 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.103 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | SHARP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.900 | 3.800 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Rmerge | 0.102 | 0.965 |
| Number of reflections | 20599 | |
| <I/σ(I)> | 7.7 | |
| Completeness [%] | 97.8 | |
| Redundancy | 2.5 | |
| CC(1/2) | 0.990 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293.15 | Peg300, 0.1 MES ph 6.5 |
