5H5N
The crystal structure of the NS1 (H17N10) RNA-binding domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-01-31 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97915 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 53.847, 53.847, 114.927 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.292 - 2.000 |
R-factor | 0.2116 |
Rwork | 0.210 |
R-free | 0.25300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ail |
RMSD bond length | 0.009 |
RMSD bond angle | 1.245 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.060 | 0.500 |
Number of reflections | 11847 | |
<I/σ(I)> | 28.6 | |
Completeness [%] | 97.8 | 98.8 |
Redundancy | 6.6 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.085M HEPES pH 7.5, 3.655M sodium chloride, 15% glycerol |