5GZO

Structure of neutralizing antibody bound to Zika envelope protein

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2016-05-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.987
Spacegroup name	C 1 2 1
格子定数 [Å]	128.668, 97.504, 165.753
格子定数 [度]	90.00, 91.52, 90.00

精密化法

残基	44.680 - 2.755
R因子	0.2334
Rwork	0.231
R-free	0.27720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jhm 5jhl
結合長の平均二乗偏差(RMSD) [Å]	0.005
結合角の平均二乗偏差(RMSD) [度]	1.074
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	50.000	2.860
分解能 [Å] (高)	2.760	2.760
独立反射数	53031
<I/σ(I)>	9.903
完全性 [%]	99.9
冗長性	5.5

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	0.12 M Alcohols, 0.1 M Buffer System 2, 50%(v/v) Precipitant Mix 2