5GZN

Structure of neutralizing antibody bound to Zika envelope protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	C 2 2 21
Unit cell lengths	124.708, 129.385, 428.711
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.080 - 3.000
R-factor	0.2243
Rwork	0.223
R-free	0.25750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jhm 5jhl
RMSD bond length	0.004
RMSD bond angle	0.847
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.110
High resolution limit [Å]	3.000	3.000
Number of reflections	69280
<I/σ(I)>	10.7
Completeness [%]	99.5
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	0.1 M potassium chloride, 0.1 M HEPES, 25%(v/v) SOKALAN CP 7