5GNF
Crystal structure of anti-CRISPR protein AcrF3
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 65 |
| 格子定数 [Å] | 84.944, 84.944, 63.155 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 42.472 - 1.500 |
| R因子 | 0.1711 |
| Rwork | 0.170 |
| R-free | 0.19190 |
| Structure solution method | SAD |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.006 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.781 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 1.550 |
| 分解能 [Å] (高) | 1.500 | 1.500 |
| Rmerge_l_obs | 0.065 | 0.403 |
| 独立反射数 | 41528 | |
| <I/σ(I)> | 43.8 | 3.4 |
| 完全性 [%] | 99.9 | 99.6 |
| 冗長性 | 15.4 | 7.1 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 289 | 20% (w/v) PEG 8000, 100mM MES, 200mM Calcium acetate |
