5FT1
Crystal structure of gp37(Dip) from bacteriophage phiKZ bound to RNase E of Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-01 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 84.610, 84.650, 84.600 |
| Unit cell angles | 107.86, 107.88, 107.90 |
Refinement procedure
| Resolution | 72.188 - 2.750 |
| R-factor | 0.266 |
| Rwork | 0.263 |
| R-free | 0.29340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ft0 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.709 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.200 | 2.840 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.030 | 0.430 |
| Number of reflections | 39553 | |
| <I/σ(I)> | 12.3 | 1.7 |
| Completeness [%] | 94.2 | 93.2 |
| Redundancy | 1.9 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100 MM KH2PO4, 100 MM NAH2PO4, 100 MM MES PH 6.0, 300 MM NACL, 0.2 M SODIUM THIOCYANATE |
